Application

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Signaling

Anti-Nalcn Antibody, clone N185/7 is a highly specific mouse monoclonal antibody & that targets Sodium leak channel non-selective protein & has been tested in western blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Nalcn in rat brain tissue.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Sodium leak channel non-selective protein (NALCN) is also called Four domain-type voltage-gated ion channel alpha-1 subunit and Voltage gated channel-like protein 1 (Vgcnl1). NALCN is a voltage-independent, cation-nonselective channel which transports sodium, potassium and calcium ions. NALCN maintains the background sodium ion leak current in neurons, controls neuronal excitability and is essential for normal respiratory rhythm and neonatal survival. NALCN is activated by substance P or neurotensin, and is mainly expressed in the brain or to a lesser extent, heart and pancreas.

Immunogen

Epitope: C terminal cytoplasmic domain

Recombinant protein corresponding to the C terminal cytoplasmic domain of rat Nalcn.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in human hippocampus membrane tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Nalcn in 10 µg of human hippocampus membrane tissue lysate.

Specificity

This antibody recognizes the C terminal cytoplasmic domain of Nalcn.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 200 kDa observed